Coding

Part:BBa_K2309022

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-26)


LL-37 with A His-Tag for Lactococcus lactis NZ9000 (codon optimized)

The separation and purification of antimicrobial peptides are important problems due to their small molecular weights and special structures. Therefore, we add a His-Tag to the C-terminal to our largest antimicrobial peptide -- LL-37 for the separation and purification by affinity chromatography. Whereafter, SDS-PAGE can be used to check the molecular weight of LL-37 and determine whether LL-37 and other peptides are successfully expressed in Lactococcus lactis NZ9000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None